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KMID : 0613820070170060822
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Journal of Life Science
2007 Volume.17 No. 6 p.822 ~ p.827
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Efficient plant regeneration from cotyledon and primary leaf explants of lettuce (Lactuca sativa L.)
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Son Bo-Hwa
Park Chul-Gyoo
Ahn Nam-Young
Jeon Joo-Mi
Kim Cha-Young
Oh Se-Chan
Lee Young-Hoon
Gal Sang-Wan
Lee Sang-Ho
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Abstract
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The efficient system for plant regeneration from cotyledon and primary leaf explants of lettuce was established. Plant regeneration efficiency was shown 91.3% from cotyledon and 85.9% from primary leaf explants of variety ’Jungtongpogi’ in KN medium. Plant regeneration efficiency was also estimated with various plant regeneration media in variety ’Chungchima’, which was lowest plant regeneration efficient showing 35.4% from cotyledon and 30.3% from primary leaf explants in KN medium. KI medium increased 77.9% and 80.7% of plant regeneration efficiencies from cotyledon and primary leaf explants of variety ’Chungchima’, respectively. Therefore, efficient plant regeneration was obtained when cotyledons of variety ’Jungtongpogi’ were cultured on KN medium. In case of variety ’Chungchima’, efficient plant regeneration was shown when primary leaf explants were cultured on SH and KI media.
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KEYWORD
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lettuce, Lactuca sativa L., Plant Regeneration, Plant Tissue Culture
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